P-72: Comparative Histopathologic Evaluation of Portulaca Oleracea, Omega-3 Fatty Acids and Combination of Sodium Selenite and Vitamin E on Testis of Experimental Diabetic Rats

Background: Portulaca oleracea (PO) plant, omega-3 fatty acids and combination of sodium selenite and vitamin E can limit negative impacts on testicular tissue and function which may be caused by compensating for defective insulin release, enhancing antioxidant status and decreasing in blood glucose levels. Materials and Methods: A total of 96 adult male Sprague Dawley (SD) rats (weighing approximately 220 ± 10 g) were injected by a single intraperitonial injection of streptozotocin (70 mg/kg Body weight.) and were randomly assigned to 4 groups and 4 replicates for each group. Group 1 served as diabetic control, group 2 received PO juice (1.5 mg/kg/day, orally), group 3 received omega-3 fatty acids (500 mg/kg/day, orally) and group 4 received vitamin E in forms of alpha-tocopheryl acetate (400 Iu/kg/day, orally) and sodium selenite (0.5 mg/kg/ day, orally) for a period of 7 days. At the end of the study period, testis samples were taken for histopathological investigations. spermatogonia, spermatocyte, spermatid and sertoli cells in all groups were calculated. Results: Reduction in number of espermatogonial cells in animals treated with combination of sodium selenite and vitamin E was less than other groups. Spermatocytes and sertoli cells in group 4 was more than other treatment groups. Number of spermatid cells in groups treated with omega-3 and combination of sodium selenite and vitamin E was more than control and PO groups. Conclusion: Treatment with combination of sodium selenite and vitamin E in male diabetic rats revealed more positive effects compared to other treatment groups.